禽呼肠孤病毒强毒株和弱毒疫苗株感染鸡肝癌细胞的转录组学分析

doi:10.11843/j.issn.0366-6964.2017.11.015

Abstract

Abstract:

To discover differential expressed key genes in LMH cells infected with ARV virulent and attenuated vaccine strain, RNA-seq sequencing was utilized to perform the transcriptomic analysis. LMH cells were infected with ARV virulent strain S1133 and attenuated strain aS1133 at the dosage of 1 MOI, the infected cells and uninfected cells were collected at 6, 12 and 24 hpi to prepare total RNA samples for transcriptomic analysis by Illumina HiSeq^TM 2000 sequencer. Genes between S1133-infected and aS1133 infected cells with expression difference times ≥ 2 and P ≤ 0.001 were set as differentially expressed genes for further analysis. The analysis results showed that compared with aS1133-infected cells, 4 013 differentially expressed genes (DEGs) were discovered, including 58 up-regulated and 5 down-regulated DEGs at 6 hpi; 1 429 up-regulated and 354 down-regulated at 12 hpi; 1 680 up-regulated and 481 down-regulated at 24 hpi. These DEGs expression modes were categorized into 10 co-expression trends. With GO functional annotation analysis, the biological functions of these DEGs were associated with GTPase regulator activity, signal transducer activity, protein kinase activity; and these DEGs were involved in response to biotic stimulus, cell apoptotic process, biological regulation, signal transduction. The pathway analysis showed that DEGS were involved in a serious of signal pathways such as Toll-like receptor signal pathway, TGF-β signal pathway. The results of qRT-PCR for detection of DEGs randomly selected were consistent with those of transcriptomic analysis. The above data compared and analyzed that at the transcription level, differentially expressed genes in LMH cells after ARV virulent and attenuated strain infection and explored the possible different responses for host cells to ARV different strains, which would contribute to further understand ARV pathogenesis.

CLC Number:

S852.659.4

HUANG Li, XIE Zhi-xun, XIE Li-ji, HUANG Jiao-ling, XIE Zhi-qin, FAN Qing, DENG Xian-wen, LUO Si-si, ZHANG Yan-fang, ZENG Ting-ting, WANG Sheng, ZHANG Min-xiu. Transcriptomic Expression Profiles of LMH Cells Infected with Avian Reovirus Virulent Strain and Attenuated Vaccine Strain[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(11): 2135-2147.

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Transcriptomic Expression Profiles of LMH Cells Infected with Avian Reovirus Virulent Strain and Attenuated Vaccine Strain

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